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Regulation of leptin distribution between plasma and cerebrospinal fluid by cholecystokinin receptors
Author(s) -
Cano Victoria,
Ezquerra Laura,
Ramos M Pilar,
RuizGayo Mariano
Publication year - 2003
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0705477
Subject(s) - leptin , endocrinology , medicine , cholecystokinin , postprandial , receptor , chemistry , cholecystokinin receptor , antagonist , endogeny , insulin , receptor antagonist , cerebrospinal fluid , hormone , leptin receptor , biology , obesity
Cholecystokinin (CCK) is a postprandial hormone that elicits a satiating effect and regulates feeding behaviour. CCK has been shown to enhance the effect of leptin in several experimental paradigms. The goal of this work was to characterize the effect of endogenous CCK on plasma leptin content by using CCK receptor antagonists. Therefore, we administered SR‐27,897, a selective CCK 1 receptor antagonist, and L‐365,260, a selective CCK 2 receptor antagonist, to fed and food‐deprived rats and determined plasma leptin concentration by enzyme immunoassay. Plasma insulin and glucose concentration as well as food intake were also determined. Under our conditions, SR‐27,897 increased plasma concentration of leptin both in fed and food‐deprived rats. It also increased food intake as well as plasma concentration of insulin in fed animals. L‐365,260 increased plasma leptin concentration only in fed rats. In animals receiving exogenous leptin, CCK‐8 increased the ratio between the concentration of leptin in cerebrospinal fluid and plasma. These results show that CCK receptor antagonists increases plasma concentration of leptin and suggest that endogenous CCK may facilitate the uptake of plasma leptin to the cerebrospinal fluid.British Journal of Pharmacology (2003) 140 , 647–652. doi: 10.1038/sj.bjp.0705477