Electrophysiological evidence for a gradient of G protein‐gated K + current in adult mouse atria

Whole cell current and voltage clamp techniques were used to examine the properties of acetylcholine‐sensitive K + current (IKACh) in myocytes from adult mouse atrium. Superfusion of a maximal dose of carbachol (CCh; 10 μ M ) caused a substantial increase in K + current in all myocytes examined. The current–voltage ( I – V ) relation of maximally activated IKACh exhibited weak inward rectification. Consequently, CCh increased the amount of depolarising current necessary to evoke action potentials (APs), and APs evoked in CCh had significantly shorter durations than control APs ( P <0.05). The effects of CCh on K + current and on AP properties were blocked by the muscarinic receptor antagonist methoctramine (1 μ M ). ACh (10 μ M ) activated a K + current with identical properties to that activated by CCh, as did the A 1 receptor agonist adenosine (100 μ M ). Right atrial myocytes had significantly more IKACh than left atrial myocytes ( P <0.05), regardless of whether IKACh was evoked by superfusion of muscarinic or A 1 receptor agonists. IKACh current density was significantly higher in SA node myocytes than either right or left atrial myocytes. These data identify a gradient of IKACh current density across the supraventricular structures of mouse heart. This gradient, combined with the heterogeneous distribution of parasympathetic innervation of the atria, may contribute to the proarrhythmic ability of vagal nerve stimulation to augment dispersion of atrial refractoriness.British Journal of Pharmacology (2003) 140 , 576–584. doi: 10.1038/sj.bjp.0705474