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Electrophysiological effects of activating the peptidergic primary afferent innervation of rat mesenteric arteries
Author(s) -
Dunn W R,
Hardy T A,
Brock J A
Publication year - 2003
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0705417
Subject(s) - capsaicin , hyperpolarization (physics) , calcitonin gene related peptide , electrophysiology , glibenclamide , chemistry , medicine , endocrinology , excitatory postsynaptic potential , stimulation , substance p , mesenteric arteries , neuropeptide , membrane potential , inhibitory postsynaptic potential , receptor , artery , biochemistry , organic chemistry , nuclear magnetic resonance spectroscopy , diabetes mellitus
Intracellular recording was used to investigate the electrophysiological effects of activating peptidergic primary afferent axons with capsaicin in the smooth muscle of rat mesenteric arteries in vitro . In addition, continuous amperometry was used to monitor the effects of capsaicin on noradrenaline release from the sympathetic nerves. Capsaicin (1 μ M ) produced a hyperpolarization (−11±2 mV) and a reduction in the time constant of decay of excitatory junction potentials (e.j.p.'s) evoked by electrical stimulation of the perivascular sympathetic nerves. These effects of capsaicin were mimicked by calcitonin gene‐related peptide (CGRP; 1 and 10 n M ) but not by substance P (50 n M ), which produced a small hyperpolarization (maximum −3±1 mV) but did not change excitatory junction potential (e.j.p.) time course. The hyperpolarization produced by capsaicin and CGRP was blocked by glibenclamide (10 μ M ) but was not changed by the CGRP antagonist, CGRP 8–37 (0.5 μ M ). Mechanical denudation of the endothelium also did not reduce the effect of capsaicin on membrane potential. Capsaicin (1 μ M ) increased the amplitude of e.j.p.'s. This effect was not mimicked by CGRP or substance P nor blocked by glibenclamide or CGRP 8–37 . All effects of capsaicin desensitized. Capsaicin (1 μ M ) had no effect on noradrenaline‐induced oxidation currents evoked by electrical stimulation, indicating that noradrenaline release was unchanged. These results suggest that CGRP released from primary afferent axons hyperpolarizes vascular smooth muscle by activating glibenclamide‐sensitive K + channels. The findings also indicate that an unknown factor released by the primary afferent axons increases e.j.p. amplitude.British Journal of Pharmacology (2003) 140 , 231–238. doi: 10.1038/sj.bjp.0705417