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Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions
Author(s) -
Minic Jasmina,
Chatonnet Arnaud,
Krejci Eric,
Molgó Jordi
Publication year - 2003
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0705010
Subject(s) - acetylcholinesterase , butyrylcholinesterase , chemistry , aché , neostigmine , muscarinic acetylcholine receptor , acetylcholine , neuromuscular junction , cholinergic , acetylcholine receptor , postsynaptic potential , receptor , pharmacology , biochemistry , endocrinology , enzyme , neuroscience , biology
The present study was performed to evaluate the presence and the physiological consequences of butyrylcholinesterase (BChE) inhibition on isolated phrenic‐hemidiaphragm preparations from normal mice expressing acetylcholinesterase (AChE) and BChE, and from AChE‐knockout mice (AChE −/− ) expressing only BChE. Histochemical and enzymatic assays revealed abundance of AChE and BChE in normal mature neuromuscular junctions (NMJs). In normal NMJs, in which release was reduced by low Ca 2+ /high Mg 2+ medium BChE inhibition with tetraisopropylpyrophosphoramide (iso‐OMPA) or bambuterol decreased (∼50%) evoked quantal release, while inhibition of AChE with fasciculin‐1, galanthamine (10, 20 μ M ) or neostigmine (0.1–1 μ M ) increased (50–80%) evoked quantal release. Inhibition of both AChE and BChE with galanthamine (80 μ M ), neostigmine (3–10 μ M ), O‐ethylS‐2‐(diisopropylamino)ethyl‐methylphosphono‐thioate (MTP) or phospholine decreased evoked transmitter release (20–50%). In AChE −/− NMJs, iso‐OMPA pre‐treatment decreased evoked release. Muscarinic toxin‐3 decreased evoked release in both AChE −/− and normal NMJs treated with low concentrations of neostigmine, galanthamine or fasciculin‐1, but had no effect in normal NMJs pretreated with iso‐OMPA, bambuterol, MTP and phospholine. In normal and AChE −/− NMJs pretreatment with iso‐OMPA failed to affect the time course of miniature endplate potentials and full‐sized endplate potentials. Overall, our results suggest that inhibition or absence of AChE increases evoked quantal release by involving muscarinic receptors (mAChRs), while BChE inhibition decreases release through direct or indirect mechanisms not involving mAChRs. BChE apparently is not implicated in limiting the duration of acetylcholine action on postsynaptic receptors, but is involved in a presynaptic modulatory step of the release process.British Journal of Pharmacology (2003) 138 , 177–187. doi: 10.1038/sj.bjp.0705010