Premium
A study on the role of nitric oxide and iron in 3‐morpholino‐sydnonimine‐induced increases in dopamine release in the striatum of freely moving rats
Author(s) -
Serra Pier Andrea,
Rocchitta Gaia,
Esposito Giovanni,
Delogu M Rosaria,
Migheli Rossana,
Miele Egidio,
Desole Maria S,
Miele Maddalena
Publication year - 2001
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0704232
Subject(s) - chemistry , deferoxamine , peroxynitrite , dopamine , sodium nitroprusside , striatum , nitric oxide , ascorbic acid , superoxide , endocrinology , medicine , biochemistry , pharmacology , enzyme , food science , organic chemistry
We showed previously that interaction between NO and iron (II), both released following the decomposition of sodium nitroprusside (SNP), accounted for the late SNP‐induced dopamine (DA) increase in dialysates from the striatum of freely moving rats; in addition, we showed that co‐infusion of iron (II) with the NO‐donor S‐nitroso‐N‐acetylpenicillamine mimicked SNP effects on striatal DA release. In the present study, intrastriatal co‐infusion of iron (II) (given as FeSO 4 , 1 m M for 40 min) with the NO‐donor and potential peroxynitrite generator 3‐morpholinosydnonimine (SIN‐1) (0.2, 0.5, 1.0 or 5.0 m M for 180 min), potentiated the SIN‐1‐induced increase in DA concentration in dialysates from the striatum of freely moving rats. Neither alone nor associated with iron (II) did SIN‐1 induce changes in dialysate ascorbic acid or uric acid concentrations. Neither co‐infusion of a superoxide dismutase mimetic nor uric acid affected SIN‐1‐induced increases in dialysate DA concentration. Infusion of the iron chelator deferoxamine (0.2 m M for 180 min) decreased dialysate DA and attenuated SIN‐1‐induced increases in dialysate DA concentrations. These results suggest that iron plays a key role in SIN‐1‐induced release of striatal DA and do not support any role for either peroxynitrite or superoxide anion in SIN‐1‐induced release of striatal DA.British Journal of Pharmacology (2001) 134 , 275–282; doi: 10.1038/sj.bjp.0704232