Effects of ACE inhibition and angiotensin II type 1 receptor blockade on cardiac function and G proteins in rats with chronic heart failure

Inhibition of the renin‐angiotensin system (RAS) improves symptoms and prognosis in heart failure. The experimental basis for these benefits remains unclear. We examined the effects of inhibition of ACE or blockade of angiotensin II type 1 (AT1) receptor on the haemodynamics, cardiac G‐proteins, and collagen synthesis of rats with coronary artery ligation (CAL), a model in which chronic heart failure (CHF) is induced. Rats were orally treated with the ACE inhibitor trandolapril (3 mg kg −1  day −1 ) or the AT1 receptor blocker L‐158809 (1 mg kg −1  day −1 ) from the 2nd to 8th week after CAL. CAL resulted in decreases in the left ventricular systolic pressure and its positive and negative dP/dt, an increase in the left ventricular end‐diastolic pressure, and the rightward shift of the left ventricular pressure‐volume curve. Long‐term treatment with either drug improved these signs of CHF to a similar degree. Cardiac Gsα and Gqα protein levels decreased, whereas the level of Giα protein increased in the animals with CHF. Long‐term treatment with trandolapril or L‐158809 attenuated the increase in the level of cardiac Giα protein of the animals with CHF without affecting Gsα and Gqα protein levels. Cardiac collagen content of the failing heart increased, whose increase was blocked by treatment with either drug. Exogenous angiotensin I stimulated collagen synthesis in cultured cardiac fibroblasts, whose stimulation was attenuated by either drug. These results suggest that blockade of the RAS, at either the receptor level or the synthetic enzyme level, may attenuate the cardiac fibrosis that occurs after CAL and thus affect the remodelling of the failing heart.British Journal of Pharmacology (2001) 134 , 150–160; doi: 10.1038/sj.bjp.0704219