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Estimation of anti‐platelet drugs on human platelet aggregation with a novel whole blood aggregometer by a screen filtration pressure method
Author(s) -
Sudo Toshiki,
Ito Hideki,
Ozeki Yasushi,
Kimura Yukio
Publication year - 2001
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0704204
Subject(s) - whole blood , cilostazol , platelet , chemistry , pharmacology , dipyridamole , sulfinpyrazone , tirofiban , platelet aggregation inhibitor , platelet rich plasma , aspirin , medicine , biochemistry , percutaneous coronary intervention , myocardial infarction
The effects of anti‐platelet drugs on human whole blood aggregation were evaluated using a novel whole blood aggregometer by a screen filtration pressure (SFP) method. The SFP whole blood aggregometer was found to successfully detect whole blood aggregation induced by ADP, collagen and TRAP by measuring the SFP of blood samples. The platelet aggregation threshold index (PATI), the concentration of agonist required with an inducing pressure rate of 50%, varied time‐dependently after collection of blood. High values for ADP and collagen were noted immediately after blood collection, suggesting low aggregation activity of platelets, and gradually increase thereafter. Cilostazol (phosphodiesterase 3 inhibitor), dipyridamole, aspirin and tirofiban all inhibited whole blood aggregation in vitro . Inhibitory effects of cilostazol and dipyridamole, but not tirofiban, were markedly enhanced 6 or 7 fold by long pre‐incubation (60 min), compared with short pre‐incubation (2 min). Such enhancement was only observed with ADP‐ and not collagen‐induced whole blood aggregation. A similar phenomenon was also observed for aggregation with platelet rich plasma (PRP). Cilostazol inhibition of ADP‐induced platelet aggregation was more potent with PRP than whole blood (PATI 200 =3.80±0.95 μ M for whole blood; 2.04±0.61 μ M for PRP). Inhibitory effects of dipyridamole were attenuated in PRP without erythrocytes. These results demonstrate that the SFP aggregometer can sensitively detect anti‐platelet aggregatory effects of various kinds of drugs. So that it is a useful tool for evaluation of anti‐platelet drugs.British Journal of Pharmacology (2001) 133 , 1396–1404; doi: 10.1038/sj.bjp.0704204