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Absence of ligand‐induced regulation of kinin receptor expression in the rabbit
Author(s) -
Sabourin Thierry,
Guay Katline,
Houle Steeve,
Bouthillier Johanne,
Bachvarov Dimcho R,
Adam Albert,
Marceau François
Publication year - 2001
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0704158
Subject(s) - kinin , kininogen , receptor , bradykinin , population , medicine , stimulation , endocrinology , in vivo , endogeny , chemistry , biology , microbiology and biotechnology , environmental health
The induction of B 1 receptors (B 1 Rs) and desensitization or down‐regulation of B 2 receptors (B 2 Rs) as a consequence of the production of endogenous kinins has been termed the autoregulation hypothesis. The latter was investigated using two models based on the rabbit: kinin stimulation of cultured vascular smooth muscle cells (SMCs) and in vivo contact system activation (dextran sulphate intravenous injection, 2 mg kg −1 , 5 h). Rabbit aortic SMCs express a baseline population of B 1 Rs that was up‐regulated upon interleukin‐1β treatment ([ 3 H]‐Lys‐des‐Arg 9 ‐BK binding or mRNA concentration evaluated by RT–PCR; 4 or 3 h, respectively). Treatment with B 1 R or B 2 R agonists failed to alter B 1 R expression under the same conditions. Despite consuming endogenous kininogen (assessed using the kinetics of immunoreactive kinin formation in the plasma exposed to glass beads ex vivo ) and producing hypotension mediated by B 2 Rs in anaesthetized rabbits, dextran sulphate treatment failed to induce B 1 Rs in conscious animals (RT–PCR in several organs, aortic contractility). By contrast, lipopolysaccharide (LPS, 50 μg kg −1 , 5 h) was an effective B 1 R inducer (kidney, duodenum, aorta) but did not reduce kininogen reserve. We tested the alternate hypothesis that endogenous kinin participate in LPS induction of B 1 Rs. Kinin receptor antagonists (icatibant combined to B‐9858, 50 μg kg −1 of each) failed to prevent or reduce the effect of LPS on B 1 R expression. Dextran sulphate or LPS treatments did not persistently down‐regulate vascular B 2 Rs (jugular vein contractility assessed ex vivo ). The kinin receptor autoregulation hypothesis is not applicable to primary cell cultures derived from a tissue known to express B 1 Rs in a regulated manner (aorta). The activation of the endogenous kallikrein‐kinin system is ineffective to induce B 1 Rs in vivo in an experimental time frame sufficient for B 1 R induction by LPS.British Journal of Pharmacology (2001) 133 , 1154–1162; doi: 10.1038/sj.bjp.0704158