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Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice
Author(s) -
Satoh Eiki,
Ishii Toshiaki,
Shimizu Yoshio,
Sawamura Shinichi,
Nishimura Masakazu
Publication year - 2001
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0703883
Subject(s) - neurotoxin , toxin , clostridium botulinum , tetrodotoxin , botulinum toxin , pharmacology , in vitro , botulism , toxicity , chemistry , biology , medicine , microbiology and biotechnology , anesthesia , biochemistry , endocrinology , organic chemistry
Botulinum neurotoxin type A (BoNT/A, 1.5 n M ) completely inhibited indirectly evoked twitches in in vitro mouse phrenic nerve‐diaphragm preparations within 40 – 45 min. Black tea extract, thearubigin fraction (TRB), mixed with BoNT/A blocked the inhibitory effect of the toxin. The protective effect of TRB extended to botulinum neurotoxins types B and E (BoNT/B and BoNT/E) and tetanus toxin, but not to tetrodotoxin. TRB was also effective against oral toxicity of BoNT/A, B and E. Thus, TRB may be of potential benefit in protecting the paralytic actions of botulinum neurotoxins (BoNTs), but its use is limited by mixing with the toxin. British Journal of Pharmacology (2001) 132 , 797–798; doi: 10.1038/sj.bjp.0703883