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Methyl p‐hydroxybenzoate (E‐218) a preservative for drugs and food is an activator of the ryanodine receptor Ca 2+ release channel
Author(s) -
Cavagna Dario,
Zorzato Francesco,
Babini Elena,
Prestipino Gianfranco,
Treves Susan
Publication year - 2000
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0703571
Subject(s) - haloperidol , activator (genetics) , ryanodine receptor , chemistry , preservative , calcium , skeletal muscle , pharmacology , endoplasmic reticulum , medicine , biochemistry , receptor , food science , dopamine , organic chemistry
Haloperidol is a drug used in the management of several psychotic disorders and its use has been linked to Neuroleptic Malignant Syndrome. In the present study we have investigated the effect of a commercial preparation of haloperidol, Serenase, on skeletal muscle sarcoplasmic reticulum. Addition of Serenase to isolated terminal cisternae caused a rapid release of calcium. We tested whether the active Ca 2+ ‐releasing substance was haloperidol or another compound present in the preparation. Our results show that methyl p‐hydroxybenzoate, one of the preservatives and a commonly used anti‐microbial agent (E‐218) is an activator of Ca 2+ release (E.C.50=2.0 m M ), mediated by a ruthenium red‐sensitive Ca 2+ release channel present in skeletal muscle terminal cisternae.British Journal of Pharmacology (2000) 131 , 335–341; doi: 10.1038/sj.bjp.0703571