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Functional group I metabotropic glutamate receptors in submucous plexus of guinea‐pig ileum
Author(s) -
Hu HongZhen,
Ren Jun,
Liu Sumei,
Gao Chuanyun,
Xia Yun,
Wood Jackie D
Publication year - 1999
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0702980
Subject(s) - metabotropic glutamate receptor , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 2 , metabotropic glutamate receptor 6 , metabotropic receptor , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 7 , submucous plexus , glutamate receptor , myenteric plexus , biology , metabotropic glutamate receptor 4 , medicine , endocrinology , neuroscience , chemistry , pharmacology , receptor , immunohistochemistry
Intracellular recording methods and immunostaining revealed the existence of functional group I metabotropic glutamate receptors (mGluRs) in submucous plexus neurons of guinea‐pig ileum. Selective group I, but not groups II or III metabotropic glutamate receptor agonists induced concentration‐dependent, slowly‐activating depolarizing responses. Group I metabotropic glutamate receptor antagonism observed with (S)‐4‐carboxyphenylglycine (S‐4‐CPG) (100–600 μ M ) was competitive as determined by Schild analysis (pA 2 =3.81±0.02). Neither the group II and III metabotropic nor ionotropic glutamate receptor antagonists altered responses evoked by group I receptor agonists. Immunoreactivities for metabotropic glutamate 1α and 5 receptors were found to locate exclusively in neurons in the submucous plexus of guinea‐pig ileum with the highest density around the cell bodies. The results suggest that group I metabotropic glutamate receptors are functionally expressed in the submucous plexus of guinea‐pig small intestine. British Journal of Pharmacology (1999) 128 , 1631–1635; doi: 10.1038/sj.bjp.0702980