Premium
Alternative splicing generates two isoforms of the β 3 ‐adrenoceptor which are differentially expressed in mouse tissues
Author(s) -
Evans B A,
Papaioannou M,
Hamilton S,
Summers R J
Publication year - 1999
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0702688
Subject(s) - splice , exon , gene isoform , intron , biology , alternative splicing , rna splicing , coding region , messenger rna , microbiology and biotechnology , white adipose tissue , adipose tissue , gene , genetics , endocrinology , rna
The β 3 ‐adrenoceptor (AR) differs from the β 1 ‐AR and β 2 ‐ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β 3 ‐AR which differ within the coding region. Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate the splice variant of the mouse β 3 ‐adrenoceptor. The novel β 3b ‐AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β 3a ‐AR (RFDGYEGARPFPT). β 3b ‐AR mRNA is differentially expressed in mouse tissues, with levels relative to β 3a ‐AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.British Journal of Pharmacology (1999) 127 , 1525–1531; doi: 10.1038/sj.bjp.0702688