Comparison of the redox forms of nitrogen monoxide with the nitrergic transmitter in the rat anococcygeus muscle

A sustained tone was produced in rat isolated anococcygeus muscles with guanethidine and clonidine and relaxant responses were elicited by electrical stimulation of its nitrergic nerves and by the three redox forms of nitrogen monoxide. The nitroxyl anion (NO − ) was donated by dissociation of Angeli's salt; the free radical (NO • ) was from an aqueous solution of nitric oxide gas; the nitrosonium cation (NO + ) was donated by dissociation of nitrosonium tetrafluoroborate. The concentrations producing approximately 50% relaxations of the anococcygeus muscle were 0.3 μ M for Angeli's salt (nitroxyl), 0.5 μ M for NO • and 100 μ M for nitrosonium tetrafluoroborate. Nitrergic nerve stimulation at 1 Hz for 10 s produced equivalent relaxant responses. The superoxide generator pyrogallol (100 μ M ) had no effect on responses to nitrergic nerve stimulation or Angeli's salt but significantly reduced responses to NO • and nitrosonium tetrafluoroborate. The NO • scavenger carboxy‐PTIO (100 μ M ) had no effect on responses to nitrergic nerve stimulation or Angeli's salt but significantly reduced responses to NO • and nitrosonium tetrafluoroborate. Hydroxocobalamin (30 μ M ) had no significant effect on responses to the nitrergic transmitter, enhanced the response to Angeli's salt, and significantly reduced responses to NO • and nitrosonium tetrafluoroborate. The findings suggest that the nitroxyl anion donated by Angeli's salt is a better candidate than NO • to serve as the nitrergic transmitter in the rat anococcygeus muscle, although it still does not behave exactly like the transmitter.British Journal of Pharmacology (1999) 127 , 826–834; doi: 10.1038/sj.bjp.0702540