Thermogenic effects of sibutramine and its metabolites

The thermogenic activity of the serotonin and noradrenaline reuptake inhibitor sibutramine (BTS 54524; Reductil) was investigated by measuring oxygen consumption (VO 2 ) in rats treated with sibutramine or its two pharmacologically‐active metabolites. Sibutramine caused a dose‐dependent rise in VO 2 , with a dose of 10 mg kg −1 of sibutramine or its metabolites producing increases of up to 30% that were sustained for at least 6 h, and accompanied by significant increases (0.5–1.0°C) in body temperature. Based on the accumulation in vivo of radiolabelled 2‐deoxy‐[ 3 H]‐glucose, sibutramine had little or no effect on glucose utilization in most tissues, but caused an 18 fold increase in brown adipose tissue (BAT). Combined high, non‐selective doses (20 mg kg −1 ) of the β‐adrenoceptor antagonists, atenolol and ICI 118551, inhibited completely the VO 2 response to sibutramine, but the response was unaffected by low, β 1 ‐adrenoceptor‐selective (atenolol) or β 2 ‐adrenoceptor‐selective (ICI 118551) doses (1 mg kg −1 ). The ganglionic blocking agent, chlorisondamine (15 mg kg −1 ), inhibited completely the VO 2 response to the metabolites of sibutramine, but had no effect on the thermogenic response to the β 3 ‐adrenoceptor‐selective agonist BRL 35135. Similar thermogenic responses were produced by simultaneous injection of nisoxetine and fluoxetine at doses (30 mg kg −1 ) that had no effect on VO 2 when injected individually. It is concluded that stimulation of thermogenesis by sibutramine requires central reuptake inhibition of both serotonin and noradrenaline, resulting in increased efferent sympathetic activation of BAT thermogenesis via β 3 ‐adrenoceptor, and that this contributes to the compound's activity as an anti‐obesity agent.British Journal of Pharmacology (1999) 126 , 1487–1495; doi: 10.1038/sj.bjp.0702446