The inhibition of nicotine‐evoked relaxation of the guinea‐pig isolated basilar artery by some analgesic drugs and progesterone

The purpose of this study was to investigate the mechanism of nicotine‐evoked relaxation of the guinea‐pig isolated basilar artery and to study the effects of drugs associated with the aetiology or treatment of migraine on the nicotine response. The guinea‐pig isolated basilar artery, pre‐contracted with prostaglandin F 2α (PGF 2α ), in the presence of atropine (3 μ M ) and guanethidine (3 μ M ), relaxed on addition of nicotine (0.1 m M ) in approximately 50% of preparations. The responses to nicotine were of short duration and blocked in preparations pre‐treated for 10 min with capsaicin (1 μ M ) and are therefore probably a consequence of the stimulation of trigeminal C fibre terminals. Responses to nicotine were reduced in the presence of 5‐carboxamidotryptamine, 5‐hydroxytryptamine and sumatriptan in that order of potency. This is consistent with a 5‐HT 1 receptor mechanism. These agonists evoked small additional contractions in vessels pre‐contracted with PGF 2α . Indomethacin (0.3–10 μ M ), aspirin (10–30 μ M ), and nitro‐ L ‐arginine methyl ester ( L ‐NAME, 0.1 m M ) reduced nicotine‐evoked relaxation of the basilar artery, suggesting the involvement of both nitric oxide and cyclo‐oxygenase products in this response. Progesterone (1 μ M ) markedly reduced the response to nicotine, a possible reflection of the ion channel blocking activity of high concentrations of this compound. The guinea‐pig basilar artery is a preparation in which the effects of drugs on responses to stimulation of trigeminal nerve terminals can be studied in vitro and may thus be of interest in assessing the actions of drugs used in treatment of headache.British Journal of Pharmacology (1999) 126 , 1003–1008; doi: 10.1038/sj.bjp.0702391