Premium
Effects of depolarizing stimuli on calcium homeostasis in cultured rat motoneurones
Author(s) -
Hubert JeanPierre,
Burgevin MarieClaude,
Terro Faraj,
Hugon Jacques,
Doble Adam
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0702207
Subject(s) - cnqx , ampa receptor , depolarization , excitotoxicity , kainate receptor , glutamate receptor , chemistry , kainic acid , calcium , riluzole , quisqualic acid , calcium in biology , nmda receptor , neuroscience , pharmacology , biophysics , biology , biochemistry , receptor , organic chemistry
1 Intracellular calcium concentrations in individual rat motoneurones in enriched primary cultures were measured by Indo‐1 fluorimetry. 2 Motoneurones in the cultures were characterized morphometrically and by cholineacetyltransferase immunocytochemistry. 3 Depolarization of the cells with glutamic acid or veratridine increased intracellular calcium levels, which returned to baseline only slowly after removal of the depolarizing agent. 4 The use of selective agonists ( N ‐methyl‐ d ‐aspartic acid, AMPA, kainic acid, quisqualic acid and 1 R ‐3 S ‐ACPD) and antagonists (MK 801 and CNQX) showed that the excitatory amino acid‐evoked responses were mediated by AMPA/kainate receptors rather than by NMDA receptors. 5 Depolarization‐evoked calcium transients in motoneurones are blocked by the neuroprotective drug riluzole 6 Calcium transients reflected entry of calcium from without the cell, and their blockade by nitrendipine and lanthanum chloride suggested that this entry took place primarily through voltage‐dependent calcium channels. 7 These findings may be relevant for understanding the selective vulnerability of motoneurones to excitotoxicity in amyotrophic lateral sclerosis, and the therapeutic activity of riluzole in the treatment of this disease.British Journal of Pharmacology (1998) 125 , 1421–1428; doi: 10.1038/sj.bjp.0702207