Effects of prenatal exposure to low concentrations of carbon monoxide on sexual behaviour and mesolimbic dopaminergic function in rat offspring

1 Inhalation of low concentrations of carbon monoxide (CO) by pregnant rats (75 and 150 p.p.m. from day 0 to day 20 of gestation) leads to changes in mesolimbic dopaminergic transmission associated with an impairment of sexual behaviour in male offspring. 2 Eighty day old males exposed in utero to CO (150 p.p.m.) exhibited a significant increase in mount/intromission latency as well as a significant decrease in mount/intromission frequency. A significant decrease in ejaculation frequency was also found in CO (150 p.p.m.)‐exposed animals. 3 The acute administration of amphetamine, at a dose (0.5 mg kg −1 s.c.) stimulating copulatory activity in control rats, failed to reduce mount/intromission latency and did not increase mount frequency in 80‐day offspring exposed to CO (150 p.p.m.) during gestation. 4 These behavioural alterations were paralleled by neurochemical changes ( in vivo microdialysis) showing that prenatal CO exposure, at concentrations (150 p.p.m.) that did not affect basal extracellular levels of dopamine in the nucleus accumbens, blunted the amphetamine (0.5 mg kg −1 s.c.)‐induced increase in dopamine release in 80‐day old male rats. 5 No significant changes in either behavioural or neurochemical parameters were observed in 10‐month old rats exposed prenatally to CO. 6 Since the alterations in sexual behaviour and dopaminergic transmission have been produced by prenatal exposure to CO levels resulting in maternal blood carboxyhaemoglobin concentrations equivalent to those maintained by human cigarette smokers, the present data further point out the large risk that the smoking mother poses for her offspring.British Journal of Pharmacology (1998) 125 , 909–915; doi: 10.1038/sj.bjp.0702143