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Evidence that cyclosporin A and dexamethasone inhibit allergic airway eosinophilic inflammation via suppression of interleukin‐5 synthesis by T cells
Author(s) -
Nakata Aya,
Kaminuma Osamu,
Mori Akio,
Ogawa Koji,
Kikkawa Hideo,
Naito Kazuaki,
Ikezawa Katsuo,
Suko Matsunobu,
Okudaira Hirokazu
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0701982
Subject(s) - ovalbumin , dexamethasone , interleukin 5 , bronchoalveolar lavage , immunology , medicine , allergic inflammation , eosinophil , inflammation , t cell , antigen , interleukin , endocrinology , immune system , cytokine , asthma , lung
1 We have recently demonstrated that airway eosinophilic inflammation can be transferred to unprimed mice by infusing interleukin (IL)‐5‐producing T cell clones. Using that murine model, we performed this study to delineate the mechanism of cyclosporin A and dexamethasone to inhibit allergic airway eosinophilic inflammation. 2 The ovalbumin‐reactive murine T cell clones, FJ17, produced IL‐2, IL‐4 and IL‐5 upon stimulation with relevant antigen. In FJ17‐transferred mice, messenger RNA (mRNA) of IL‐2 and IL‐5 expressed in the lungs, the number of eosinophils in bronchoalveolar lavage fluid (BALF) was increased and the bronchial responsiveness to acetylcholine was enhanced after antigen provocation. 3 Cyclosporin A (10, 100 ng ml −1 ) and dexamethasone (10, 100 ng ml −1 suppressed the production of IL‐5 as well as IL‐2 and IL‐4 by FJ17 in vitro . 4 Subcutaneously administered cyclosporin A (30 mg kg −1 ) and dexamethasone (10 mg kg −1 ) inhibited antigen‐induced mRNA expression of IL‐2 and IL‐5, increase of BALF eosinophils and bronchial hyperresponsiveness of FJ17‐transferred mice in vivo . The number of BALF eosinophils was correlated with the bronchial responsiveness to acetylcholine ( r = 0.672). 5 The results clearly indicated that the suppression of IL‐5 synthesis by T cells is involved in the effects of cyclosporin A and dexamethasone to inhibit allergic airway eosinophilic inflammation.British Journal of Pharmacology (1998) 124 , 1425–1432; doi: 10.1038/sj.bjp.0701982