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Culture substrate‐specific expression of P2Y 2 receptors in distal lung epithelial cells isolated from foetal rats
Author(s) -
Clunes M T.,
Collett A,
Baines D L.,
Bovell D L.,
Murphie H,
Inglis S K.,
McAlroy H L.,
Olver R E.,
Wilson S M.
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0701942
Subject(s) - receptor , lung , biology , p2y receptor , microbiology and biotechnology , messenger rna , epithelium , phenotype , chemistry , medicine , purinergic receptor , gene , biochemistry , genetics
ATP and UTP did not evoke [Ca 2+ ] i signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y 2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y 2 receptor expression thus appears to be a feature of the polarised phenotype.