z-logo
Premium
Role of adventitial nitric oxide in vascular hyporeactivity induced by lipopolysaccharide in rat aorta
Author(s) -
Kleschyov Andrei L,
Muller Bernard,
Schott Christa,
Stoclet JeanClaude
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0701916
Subject(s) - nitric oxide , aorta , nitric oxide synthase , lipopolysaccharide , chemistry , medicine
This study was designed to elucidate the role of the adventitia in NO‐mediated vascular effects of lipopolysaccharide (LPS). After incubation of rat aorta with LPS, the adventitia generated 3.5 times more nitrite plus nitrate than a corresponding segment of media. Control media covered by adventitia from LPS‐treated aortic rings exhibited a 4 fold elevated level of cyclic GMP. Medial layers from LPS‐treated aortic rings (like LPS‐treated adventitia‐intact rings) exhibited a decrease in sensitivity to noradrenaline (NA) that was reversed by 1H‐(1,2,4)oxadiazolo(4,3‐a)quinoxalin‐1‐one (1 μ M ) or N ω ‐nitro‐ L ‐arginine methylester (0.3 m M ). However, in contrast to LPS‐treated adventitia‐intact rings, medial layers showed no reduction in maximal contraction to NA and virtually no relaxation to L ‐arginine. These data indicate that in blood vessels exposed to LPS, the adventitia is a more powerful source of NO than the media. The adventitia‐derived NO can reach soluble guanylyl cyclase in the medial layer and contribute greatly to vascular hyporeactivity and L ‐arginine‐induced relaxation observed in blood vessels exposed to LPS.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here