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Impairment in connective tissue mast cells degranulation in spontaneously hypertensive rats: stimulus dependent resistance
Author(s) -
Kwasniewski F H,
Tavares de Lima W,
Bakhle Y S,
Jancar S
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0701875
Subject(s) - extravasation , degranulation , evans blue , peritoneal cavity , medicine , endocrinology , sensitization , peritoneum , vascular permeability , immunology , chemistry , pathology , anatomy , receptor
Microvascular permeability in the mesentery and consequent leakage of protein into the peritoneum of spontaneously hypertensive rats (SHR) and normotensive rats (NTR) was measured in vivo by the extravasation of Evans blue dye. In sensitized NTR, challenge with antigen produced extensive increases in dye extravasation in the mesentery and in peritoneal lavage fluid within 10 min. In sensitized SHR there was no increase in the permeability of the mesentery and a very weak increase in dye extravasation in the peritoneal cavity following challenge. The glucocorticoid antagonist RU38486 did not change the permeability response induced by antigen in sensitized NTR and SHR. However, compound 48/80 was equally effective in either NTR or SHR in causing increased vasopermeability. Mesenteric mast cells in the NTR were degranulated after immunological challenge, whereas those in the SHR were resistant, as measured histologically. Similarly, challenge ex vivo of mesentery from sensitized NTR induced contraction of guinea‐pig ileum in co‐incubation experiments, whereas SHR mesentery was unresponsive. Plasma levels of antigen‐specific IgE and IgG2a in sensitized NTR and SHR were identical. Immune serum from SHR was unable to induce a passive cutaneous anaphylaxis (PCA) reaction in the skin of NTR and SHR did not develop a PCA reaction upon passive sensitization with NTR immune serum. We conclude that the mast cells of SHR are resistant to degranulation following immunological challenge, although the relevant antibodies are present.