Effect of the triaminopyridine flupirtine on calcium uptake, membrane potential and ATP synthesis in rat heart mitochondria

1 Flupirtine is an analgesic agent which exhibits neuronal cytoprotective activity and may have value in the treatment of conditions involving cell injury and apoptosis. Since flupirtine has no action on known receptor sites we have investigated the effect of this drug on mitochondrial membrane potential, and the changes in intramitochondrial calcium concentration in particular. 2 The findings show that flupirtine increases Ca 2+ uptake in mitochondria in vitro . At clinically relevant flupirtine concentrations, corresponding to flupirtine levels in vitro of 0.2 to 10 nmol mg −1 mitochondrial protein, there was a 2 to 3 fold increase in mitochondrial calcium levels ( P <0.01). At supra‐physiological flupirtine concentrations of 20 nmol mg −1 mitochondrial protein and above, the mitochondrial calcium concentrations were indistinguishable from those in untreated mitochondria. 3 Mitochondrial membrane potential closely paralleled the changes in mitochondrial calcium levels showing a 20% ( P <0.01) increase when the flupirtine concentration was raised from 0.2 nmol to 10 nmol mg −1 mitochondrial protein and a return to control values at 20 nmol mg −1 protein. 4 The increase in mitochondrial calcium uptake and membrane potential were accompanied by an increase in mitochondrial ATP synthesis (30%; P <0.05) and a similar percentage reduction in mitochondrial volume. 5 Calcium at 80 and 160 nmol mg −1 mitochondrial protein decreased ATP synthesis by 20–25% ( P <0.001). This decrease was prevented or diminished if flupirtine at 10 nmol mg −1 protein was added before the addition of calcium. 6 Since intracellular levels of flupirtine in intact cells never exceeded 10 nmol mg −1 mitochondrial protein, these findings are supportive evidence for an in vivo cytoprotective action of flupirtine at the mitochondrial level.British Journal of Pharmacology (1998) 123 , 1154–1158; doi: 10.1038/sj.bjp.0701736