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The effect of prolonged treatment with imipramine on the biosynthesis and functional characteristics of D 2 dopamine receptors in the rat caudate putamen
Author(s) -
DziedzickaWasylewska Marta,
Rogoż Renata
Publication year - 1998
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0701681
Subject(s) - imipramine , quinpirole , agonist , dopamine , endocrinology , medicine , dopamine receptor , dopamine receptor d2 , receptor , chemistry , dopamine receptor d3 , radioligand , pharmacology , biology , alternative medicine , pathology
1 The present study shows the effects of imipramine in a single dose (10 mg kg −1 , p.o.) or following repeated (14 days, twice a day) treatment on the level of mRNA coding for D 2 dopamine receptors in the rat caudate putamen (CP). Repeated administration of imipramine resulted in the increase of the level of mRNA coding for D 2 dopamine receptors. 2 Radioligand binding studies with the D 2 receptor agonist, [ 3 H]‐N‐0437, indicated, that following imipramine administration, the affinity of the agonist for the D 2 dopamine receptor significantly increased, though without any alterations in the B max . 3 Pharmacological manipulations (by use of forskolin, GppNHp and quinpirole) of the cyclic AMP generating system, ex vivo following administration of imipramine indicated that an up‐regulation of factors inhibiting cyclic GMP formation takes place. 4 Most probably it is the D 2 dopamine receptor which undergoes functional up‐regulation, resulting from the enhancement of its biosynthesis.British Journal of Pharmacology (1998) 123 , 833–838; doi: 10.1038/sj.bjp.0701681