Open AccessGenome wide in vivo mouse screen data from studies to assess host regulation of metastatic colonisation
Author(s) -
Louise van der Weyden,
Natasha A. Karp,
A Swiaţkowska,
David J. Adams,
Anneliese O. Speak
Publication year - 2017
Publication title -
scientific data
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.565
H-Index - 64
ISSN - 2052-4463
DOI - 10.1038/sdata.2017.129
Subject(s) - colonisation , biology , metastasis , metastatic melanoma , genome , stroma , haematopoiesis , gene , cancer research , colonization , cancer , melanoma , genetics , immunology , stem cell , microbiology and biotechnology , immunohistochemistry
The process of metastasis is a multi-stage cascade with prior studies suggesting that the colonisation of the secondary site is the rate limiting step. This process involves contributions from the tumour cells and also non-tumour intrinsic factors such as the stroma and the haematopoietic system. In this study, we present data from screening 810 genetically-modified mouse lines with the experimental metastasis assay where intravenous delivery of murine metastatic melanoma B16-F10 cells was used to assess the formation of pulmonary metastasic foci. To date, these data have been studied with a two-step process cumulating in an integrative data analysis to identify genes controlling metastatic colonisation. We present the raw data, and a description to support fresh analyses where researchers can look both within and across gene sets to further elucidate process that regulate metastatic colonisation.