Open AccessCombinatory annotation of cell membrane receptors and signalling pathways of Bombyx mori prothoracic glands
Author(s) -
Panagiotis Moulos,
Martina Samiotaki,
George Panayotou,
Skarlatos Dedos
Publication year - 2016
Publication title -
scientific data
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.565
H-Index - 64
ISSN - 2052-4463
DOI - 10.1038/sdata.2016.73
Subject(s) - prothoracic gland , biology , bombyx mori , transcriptome , receptor , bombyx , microbiology and biotechnology , genome , signal transduction , ecdysone , gene , nuclear receptor , computational biology , genetics , gene expression , transcription factor
The cells of prothoracic glands (PG) are the main site of synthesis and secretion of ecdysteroids, the biochemical products of cholesterol conversion to steroids that shape the morphogenic development of insects. Despite the availability of genome sequences from several insect species and the extensive knowledge of certain signalling pathways that underpin ecdysteroidogenesis, the spectrum of signalling molecules and ecdysteroidogenic cascades is still not fully comprehensive. To fill this gap and obtain the complete list of cell membrane receptors expressed in PG cells, we used combinatory bioinformatic, proteomic and transcriptomic analysis and quantitative PCR to annotate and determine the expression profiles of genes identified as putative cell membrane receptors of the model insect species, Bombyx mori , and subsequently enrich the repertoire of signalling pathways that are present in its PG cells. The genome annotation dataset we report here highlights modules and pathways that may be directly involved in ecdysteroidogenesis and aims to disseminate data and assist other researchers in the discovery of the role of such receptors and their ligands.