z-logo
open-access-imgOpen Access
Profiling senescent cells in human brains reveals neurons with CDKN2D/p19 and tau neuropathology
Author(s) -
Shiva Kazempour Dehkordi,
Jamie M. Walker,
Eric Sah,
Emma Bennett,
Farzaneh Atrian,
Bess Frost,
Benjamin Woost,
Rachel E. Bennett,
Timothy C. Orr,
Yingyue Zhou,
Prabhakar S. Andhey,
Marco Colonna,
Peter H. Sudmant,
Peng Xu,
Minghui Wang,
Bin Zhang,
Habil Zare,
Miranda E. Orr
Publication year - 2021
Publication title -
nature aging
Language(s) - English
Resource type - Journals
ISSN - 2662-8465
DOI - 10.1038/s43587-021-00142-3
Subject(s) - senescence , phenotype , biology , lipofuscin , neurodegeneration , transcriptome , population , microbiology and biotechnology , neuropathology , human brain , neuroscience , pathology , gene expression , gene , disease , medicine , genetics , biochemistry , environmental health
Senescent cells contribute to pathology and dysfunction in animal models 1 . Their sparse distribution and heterogenous phenotype have presented challenges for detecting them in human tissues. We developed a senescence eigengene approach to identify these rare cells within large, diverse populations of postmortem human brain cells. Eigengenes are useful when no single gene reliably captures a phenotype, like senescence; they also help to reduce noise, which is important in large transcriptomic datasets where subtle signals from low-expressing genes can be lost. Each of our eigengenes detected ~2% senescent cells from a population of ~140,000 single nuclei derived from 76 postmortem human brains with various levels of Alzheimer's disease (AD) pathology. More than 97% of the senescent cells were excitatory neurons and overlapped with tau-containing neurofibrillary tangles (NFTs). Cyclin dependent kinase inhibitor 2D ( CDKN2D/ p19) was predicted as the most significant contributor to the primary senescence eigengene. RNAscope and immunofluorescence confirmed its elevated expression in AD brain tissue whereby p19-expressing neurons had 1.8-fold larger nuclei and significantly more cells with lipofuscin than p19-negative neurons. These hallmark senescence phenotypes were further elevated in the presence of NFTs. Collectively, CDKN2D/ p19-expressing neurons with NFTs represent a unique cellular population in human AD with a senescence phenotype. The eigengenes developed may be useful in future senescence profiling studies as they accurately identified senescent cells in snRNASeq datasets and predicted biomarkers for histological investigation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here