Open AccessGD2-specific CAR T cells encapsulated in an injectable hydrogel control retinoblastoma and preserve vision
Author(s) -
Kai Wang,
Yuhui Chen,
Sarah Ahn,
Min Zheng,
Elisa Landoni,
Gianpietro Dotti,
Barbara Savoldo,
Zongchao Han
Publication year - 2020
Publication title -
nature cancer
Language(s) - English
Resource type - Journals
ISSN - 2662-1347
DOI - 10.1038/s43018-020-00119-y
Subject(s) - retinoblastoma , chimeric antigen receptor , cancer research , ganglioside , retinal , medicine , chemistry , immunology , immunotherapy , ophthalmology , immune system , biochemistry , gene
Retinoblastoma (RB) is a pediatric retinal tumor that overexpresses the ganglioside GD2. Although it is treatable in patients with early diagnosis, patients may lose one or two eyes. We generated GD2-specific chimeric antigen receptor T lymphocytes (GD2.CAR-Ts) and locally delivered them to mice with an in-situ grafting RB. When used in combination with the local release of interleukin (IL)-15 and an injectable hydrogel, we showed that GD2.CAR-Ts successfully eliminate RB tumor cells without impairment of the mouse vision.