Open AccessUntargeted metabolomics links glutathione to bacterial cell cycle progression
Author(s) -
Johannes Hartl,
Patrick Kiefer,
Andreas Kaczmarczyk,
Maximilian Mittelviefhaus,
Fabian Meyer,
Thomas Vonderach,
Bodo Hattendorf,
Urs Jenal,
Julia A. Vorholt
Publication year - 2020
Publication title -
nature metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.834
H-Index - 22
ISSN - 2522-5812
DOI - 10.1038/s42255-019-0166-0
Subject(s) - caulobacter crescentus , cell cycle , metabolome , biology , microbiology and biotechnology , metabolomics , glutathione , cell division , cell growth , cell , biochemistry , bioinformatics , enzyme
Cell cycle progression requires the coordination of cell growth, chromosome replication, and division. Consequently, a functional cell cycle must be coupled with metabolism. However, direct measurements of metabolome dynamics remained scarce, in particular in bacteria. Here, we describe an untargeted metabolomics approach with synchronized Caulobacter crescentus cells to monitor the relative abundance changes of ~400 putative metabolites as a function of the cell cycle. While the majority of metabolite pools remains homeostatic, ~14% respond to cell cycle progression. In particular, sulfur metabolism is redirected during the G1-S transition, and glutathione levels periodically change over the cell cycle with a peak in late S phase. A lack of glutathione perturbs cell size by uncoupling cell growth and division through dysregulation of KefB, a K + /H + antiporter. Overall, we here describe the impact of the C. crescentus cell cycle progression on metabolism, and in turn relate glutathione and potassium homeostasis to timely cell division.