Open AccessProduction of synthetic wheat lines to exploit the genetic diversity of emmer wheat and D genome containing Aegilops species in wheat breeding
Author(s) -
Ghader Mirzaghaderi,
Zinat Abdolmalaki,
Rahman Ebrahimzadegan,
Farshid Bahmani,
Fatemeh Orooji,
Mohammad Majdi,
Ali-Akbar Mozafari
Publication year - 2020
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-020-76475-7
Subject(s) - aegilops tauschii , aegilops , biology , common wheat , triticum turgidum , genome , agronomy , ploidy , cultivar , botany , gene , genetics , chromosome
Due to the accumulation of various useful traits over evolutionary time, emmer wheat ( Triticum turgidum subsp. dicoccum and dicoccoides , 2 n = 4 x = 28; AABB), durum wheat ( T. turgidum subsp. durum , 2 n = 4 x = 28; AABB), T. timopheevii (2 n = 4 x = 28; AAGG) and D genome containing Aegilops species offer excellent sources of novel variation for the improvement of bread wheat ( T. aestivum L., AABBDD). Here, we made 192 different cross combinations between diverse genotypes of wheat and Aegilops species including emmer wheat × Ae. tauschii (2 n = DD or DDDD), durum wheat × Ae. tauschii , T. timopheevii × Ae. tauschii , Ae. crassa × durum wheat, Ae. cylindrica × durum wheat and Ae. ventricosa × durum wheat in the field over three successive years. We successfully recovered 56 different synthetic hexaploid and octaploid F 2 lines with AABBDD, AABBDDDD, AAGGDD, D 1 D 1 X cr X cr AABB, D c D c C c C c AABB and D v D v N v N v AABB genomes via in vitro rescue of F 1 embryos and spontaneous production of F 2 seeds on the F l plants. Cytogenetic analysis of F 2 lines showed that the produced synthetic wheat lines were generally promising stable amphiploids. Contribution of D genome bearing Aegilops and the less-investigated emmer wheat genotypes as parents in the crosses resulted in synthetic amphiploids which are a valuable resource for bread wheat breeding.