Open AccessCdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
Author(s) -
Tereza Vaněčková,
Jaroslava Bezděková,
Michaela Tvrdoňová,
Marcela Vlčnovská,
Veronika Novotná,
Jan Neuman,
Aneta Stossova,
Viktor Kanický,
Vojtěch Adam,
Markéta Vaculovičová,
Tomáš Vaculovič
Publication year - 2019
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-019-48290-2
Subject(s) - quantum dot , molecularly imprinted polymer , immunoassay , materials science , laser ablation , chemistry , polymerization , laser , polymer , chromatography , analytical chemistry (journal) , nanotechnology , selectivity , antibody , optics , biochemistry , physics , immunology , biology , catalysis , composite material
For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-antibody and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10–250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.