Open AccessMuscle satellite cells and impaired late stage regeneration in different murine models for muscular dystrophies
Author(s) -
A.F. Ribeiro,
Lucas Santos Souza,
Camila de Almeida,
Renata Ishiba,
Stephanie Fernandes,
Danielle A. Guerrieri,
André L. F. Santos,
P.C.G. Onofre-Oliveira,
Mariz Vainzof
Publication year - 2019
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-019-48156-7
Subject(s) - regeneration (biology) , degeneration (medical) , muscular dystrophy , mdx mouse , biology , duchenne muscular dystrophy , microbiology and biotechnology , myocyte , muscle weakness , satellite , anatomy , pathology , skeletal muscle , medicine , dystrophin , genetics , aerospace engineering , engineering
Satellite cells (SCs) are the main muscle stem cells responsible for its regenerative capacity. In muscular dystrophies, however, a failure of the regenerative process results in muscle degeneration and weakness. To analyze the effect of different degrees of muscle degeneration in SCs behavior, we studied adult muscle of the dystrophic strains: DMD mdx , Large myd , DMD mdx / Large myd , with variable histopathological alterations. Similar results were observed in the dystrophic models, which maintained normal levels of PAX7 expression, retained the Pax7-positive SCs pool, and their proliferation capacity. Moreover, elevated expression of MYOG, an important myogenic factor, was also observed. The ability to form new fibers was verified by the presence of dMyHC positive regenerating fibers. However, those fibers had incomplete maturation characteristics, such as small and homogenous fiber caliber, which could contribute to their dysfunction. We concluded that dystrophic muscles, independently of their degeneration degree, retain their SCs pool with proliferating and regenerative capacities. Nonetheless, the maturation of these new fibers is incomplete and do not prevent muscle degeneration. Taken together, these results suggest that the improvement of late muscle regeneration should better contribute to therapeutic approaches.