Open AccessAntimicrobial capacity of Leucocyte-and Platelet Rich Fibrin against periodontal pathogens
Author(s) -
Ana Belén Salamanca Castro,
Esteban Rodríguez Herrero,
Vera Slomka,
Nelson Pinto,
Wim Teughels,
Marc Quirynen
Publication year - 2019
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-019-44755-6
Subject(s) - exudate , platelet rich fibrin , porphyromonas gingivalis , antimicrobial , microbiology and biotechnology , fibrin , bacteria , agar , chemistry , biology , immunology , botany , genetics
Various studies have described the biological properties of the Leucocyte- and Platelet Rich Fibrin (L-PRF) such as the antimicrobial effect against wound bacteria, but less is known about the effect against periodontal pathogens. The aim of this study was to evaluate the antibacterial properties of the L-PRF membrane and L-PRF exudate against the main periopathogens cultured on agar plates and in planktonic solution. This study demonstrated the antibacterial effect of the L-PRF membrane against P . intermedia , F . nucleatum , and A . actinomycetemcomitans , but especially against P . gingivalis . The L-PRF exudate also showed a strong inhibition against P . gingivalis on agar plates. No inhibition could be observed for the other bacterial strains. Moreover, L-PRF exudate decreased the number of viable P . gingivalis in a planktonic solution in a dose-dependent way. However, A . actinomycetemcomitans showed an increased growth in planktonic solution when in contact with the L-PRF exudate.