Open AccessGenomic Features, Comparative Genomics, and Antimicrobial Susceptibility Patterns of Elizabethkingia bruuniana
Author(s) -
Jiun-g Lin,
Chung-Hsu Lai,
Chih-Hui Yang,
Yi-Han Huang,
HsiangWen Lin
Publication year - 2019
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-019-38998-6
Subject(s) - genome , biology , genetics , comparative genomics , whole genome sequencing , gene , genomics
Elizabethkingia bruuniana is a novel species of the Elizabethkingia genus. There is scant information on this microorganism. Here, we report the whole-genome features and antimicrobial susceptibility patterns of E . bruuniana strain EM798-26. Elizabethkingia strain EM798-26 was initially identified as E . miricola . This isolate contained a circular genome of 4,393,011 bp. The whole-genome sequence-based phylogeny revealed that Elizabethkingia strain EM798-26 was in the same group of the type strain E . bruuniana G0146 T . Both in silico DNA-DNA hybridization and average nucleotide identity analysis clearly demonstrated that Elizabethkingia strain EM798-26 was a species of E . bruuniana . The pan-genome analysis identified 2,875 gene families in the core genome and 5,199 gene families in the pan genome of eight publicly available E . bruuniana genome sequences. The unique genes accounted for 0.2–12.1% of the pan genome in each E . bruuniana . A total of 59 potential virulence factor homologs were predicted in the whole-genome of E . bruuniana strain EM798–26. This isolate was nonsusceptible to multiple antibiotics, but susceptible to aminoglycosides, minocycline, and levofloxacin. The whole-genome sequence analysis of E . bruuniana EM798-26 revealed 29 homologs of antibiotic resistance-related genes. This study presents the genomic features of E . bruuniana . Knowledge of the genomic characteristics provides valuable insights into a novel species.