Open AccessIntrogression of a functional epigenetic OsSPL14WFP allele into elite indica rice genomes greatly improved panicle traits and grain yield
Author(s) -
Sung-Ryul Kim,
Joie Ramos,
Rona Joy M. Hizon,
Motoyuki Ashikari,
P. S. Virk,
Edgar Torres,
Eero Nissilä,
Kshirod K. Jena
Publication year - 2018
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-018-21355-4
Subject(s) - panicle , cultivar , allele , biology , introgression , agronomy , horticulture , genetics , gene
Rice yield potential has been stagnant since the Green Revolution in the late 1960s, especially in tropical rice cultivars. We evaluated the effect of two major genes that regulate grain number, Gn1a/OsCKX2 and IPA1/WFP/OsSPL14 , in elite indica cultivar backgrounds. The yield-positive Gn1a -type 3 and OsSPL14 WFP alleles were introgressed respectively through marker-assisted selection (MAS). The grain numbers per panicle (GNPP) were compared between the recipient allele and the donor allele groups using segregating plants in BC 3 F 2 and BC 3 F 3 generations. There was no significant difference in GNPP between the two Gn1a alleles, suggesting that the Gn1a -type 3 allele was not effective in indica cultivars. However, the OsSPL14 WFP allele dramatically increased GNPP by 10.6–59.3% in all four different backgrounds across cropping seasons and generations, indicating that this allele provides strong genetic gain to elite indica cultivars. Eventually, five high-yielding breeding lines were bred using the OsSPL14 WFP allele by MAS with a conventional breeding approach that showed increased grain yield by 28.4–83.5% (7.87–12.89 t/ha) vis-à-vis the recipient cultivars and exhibited higher yield (~64.7%) than the top-yielding check cultivar, IRRI 156 (7.82 t/ha). We demonstrated a strong possibility to increase the genetic yield potential of indica rice varieties through allele mining and its application.