Open AccessOsSIZ2 exerts regulatory influences on the developmental responses and phosphate homeostasis in rice
Author(s) -
Wenxia Pei,
Ajay Jain,
Yuchun Sun,
Zhantian Zhang,
Hao Ai,
Xiuli Liu,
Huadun Wang,
Bing Feng,
Rui Sun,
Hongmin Zhou,
Guohua Xu,
Shubin Sun
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-10274-5
Subject(s) - mutant , gene knockdown , biology , ubiquitin ligase , oryza sativa , homeostasis , rna interference , gene , microbiology and biotechnology , gene silencing , regulation of gene expression , gene expression , genetics , ubiquitin , rna
OsSIZ1 , a small ubiquitin-related modifier (SUMO) E3 ligase, exerts regulatory influences on the developmental responses and phosphate (Pi) homeostasis in rice ( Oryza sativa ). Whether paralogs OsSIZ1 and OsSIZ2 are functionally redundant or the latter regulates these traits independent of the former is not known. To determine this, in this study, OsSIZ 2 was functionally characterized by employing reverse genetic approaches. Although the relative expression of OsSIZ 2 was spatiotemporally regulated, it showed constitutive expression in root and leaf blade irrespective of Pi regime. Analysis of T-DNA insertion knockout ( ossiz2 ) and RNAi-mediated knockdown (Ri1-3) mutants revealed positive influences on growth and developmental responses including yield-related traits. On the contrary, these mutants exhibited negative effects on the concentrations of Pi and total P in different tissues. The relative expression levels of some of the genes that are involved in Pi sensing and signaling cascades were differentially modulated in the mutants. Further, attenuation in the expression levels of OsSIZ2 in the roots of ossiz1 and relatively similar trend of the effects of the mutation in OsSIZ1 and OsSIZ2 on growth and development and total P concentration in different tissues suggested a prevalence of partial functional redundancy between these paralogs.