Open AccessFLO5 gene controls flocculation phenotype and adhesive properties in a Saccharomyces cerevisiae sparkling wine strain
Author(s) -
Paola Di Gianvito,
Catherine Tesnière,
Giovanna Suzzi,
Bruno Blondin,
Rosanna Tofalo
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-09990-9
Subject(s) - saccharomyces cerevisiae , flocculation , gene , strain (injury) , biology , yeast , phenotype , genetics , wine , chemistry , food science , organic chemistry , anatomy
Flocculation is an important feature for yeast survival in adverse conditions. The natural diversity of flocculating genes in Saccharomyces cerevisiae can also be exploited in several biotechnological applications. Flocculation is mainly regulated by the expression of genes belonging to the FLO family. These genes have a similar function, but their specific contribution to flocculation ability is still unclear. In this study, the distribution of FLO 1, FLO 5 and FLO 8 genes in four S. cerevisiae wine strains was investigated. Subsequently, both FLO 1 and FLO 5 genes were separately deleted in a flocculent S. cerevisiae wine strain. After gene disruption, flocculation ability and agar adhesion were evaluated. FLO 1 and FLO 5 genes inheritance was also monitored. All strains presented different lengths for FLO 1 and FLO 5 genes. Results confirm that in S. cerevisiae strain F6789, the FLO 5 gene drives flocculation and influences adhesive properties. Flocculation ability monitoring after a cross with a non-flocculent strain revealed that FLO 5 is the gene responsible for flocculation development.