Open AccessElectrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
Author(s) -
YiFang Tsai,
Wen-I Luo,
JenLin Chang,
Cheng-Chun Chang,
Huai-Chun Chuang,
R. Ramu,
GuorTzo Wei,
JyhMyng Zen,
Shengxue Yu
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-08610-w
Subject(s) - alkb , pseudomonas putida , chemistry , rubredoxin , monooxygenase , alkane , combinatorial chemistry , photochemistry , catalysis , organic chemistry , biochemistry , cytochrome p450 , enzyme , dna , dna repair
An unprecedented method for the efficient conversion of C 3 –C 12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (AlkB) from Pseudomonas putida GPo1 is demonstrated. The X-ray absorption spectroscopy (XAS) studies support that electrons can be transferred from the reduced AlkG (rubredoxin-2, the redox partner of AlkB) to AlkB in a two-phase manner. Based on this observation, an approach for the electrocatalytic conversion from alkanes to alcohols mediated by AlkB using an AlkG immobilized screen-printed carbon electrode (SPCE) is developed. The framework distortion of AlkB–AlkG adduct on SPCE surface might create promiscuity toward gaseous substrates. Hence, small alkanes including propane and n -butane can be accommodated in the hydrophobic pocket of AlkB for C–H bond activation. The proof of concept herein advances the development of artificial C–H bond activation catalysts.