Open AccessCombination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
Author(s) -
Weijun Kong,
Shanshan Zhang,
Yan Zhao,
Mingquan Wu,
Ping Chen,
Xuehong Wu,
Xin-Ping Ma,
Weiying Guo,
Meihua Yang
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-08377-0
Subject(s) - fingerprint (computing) , chemistry , principal component analysis , pseudomonas aeruginosa , chromatography , biology , mathematics , bacteria , computer science , artificial intelligence , statistics , genetics
The aim of this study was to explore the possible antibacterial components of Salvia miltiorrhizae on Pseudomonas aeruginosa using a combination of chemical fingerprint and bioactivity evaluation. The chemical fingerprints of 32 batches of S . miltiorrhizae samples from different sources were developed using high-performance liquid chromatography with diode array detection, and then were evaluated by similarity analysis and hierarchical clustering analysis. Anti- P . aeruginosa activity was determined by microcalorimetry. Some crucial thermokinetic parameters obtained from the heat-flow power-time curves of P . aeruginosa growth in the absence or presence of these S . miltiorrhizae samples were evaluated using principal component analysis. Thereafter, multiple linear regression analysis was used to analyze the fingerprint-activity relationship between the chemical fingerprints and anti- P . aeruginosa activity. This established the related equation between the inhibition ratio ( I , %) of S . miltiorrhizae samples on P . aeruginosa and the peak areas of the common peaks. The results showed that the 32 S . miltiorrhizae samples could be grouped into three clusters according to their chemical fingerprints and anti- P . aeruginosa activities. Protocatechualdehyde, salvianolic acid B, together with three unidentified compounds might be the major components that contributed largely to the antibacterial properties of S . miltiorrhizae and should be the focus of S . miltiorrhizae quality control. Thus, this study provided a preferred way for exploring the bioactive components of medicinal plants.