Open AccessTranscription factor ZNF148 is a negative regulator of human muscle differentiation
Author(s) -
Jesse Bakke,
William C. Wright,
Anthony E. Zamora,
Su Sien Ong,
Yue Ming Wang,
Jessica D. Hoyer,
Christopher Brewer,
Paul G. Thomas,
Taosheng Chen
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-08267-5
Subject(s) - myod , mef2c , mef2 , downregulation and upregulation , transcription factor , myosin , microbiology and biotechnology , biology , myogenesis , enhancer , myocyte , cellular differentiation , regulator , phenotype , progenitor , progenitor cell , genetics , stem cell , gene
Muscle differentiation is a complex process in which muscle progenitor cells undergo determination and eventually cellular fusion. This process is heavily regulated by such master transcription factors as MYOD and members of the MEF2 family. Here, we show that the transcription factor ZNF148 plays a direct role in human muscle cell differentiation. Downregulation of ZNF148 drives the formation of a muscle phenotype with rapid expression of myosin heavy chain, even in proliferative conditions. This phenotype was most likely mediated by the robust and swift upregulation of MYOD and MEF2C.