Open AccessAntibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
Author(s) -
Yu-Cheng Lai,
Yung Chun Chuang,
Ching Chuan Liu,
Tzong Shiann Ho,
Yee Shin Lin,
Robert Anderson,
Trai Ming Yeh
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-07308-3
Subject(s) - virology , dengue fever , dengue virus , dengue vaccine , molecular mimicry , epitope , biology , antibody , monoclonal antibody , immune system , peptide , immunization , virus , microbiology and biotechnology , immunology , biochemistry
Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cells in vitro . Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.