Open AccessTGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
Author(s) -
Kwan-Leong Hau,
Anna Maria Ranzoni,
Filipa Vlahova,
Kate Hawkins,
Paolo De Coppi,
Anna L. David,
Pascale V. Guillot
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-06780-1
Subject(s) - microbiology and biotechnology , mesenchymal stem cell , embryonic stem cell , amniotic fluid , stem cell , adenosine , downregulation and upregulation , transforming growth factor , extracellular , chemistry , population , biology , biochemistry , fetus , medicine , genetics , pregnancy , environmental health , gene
The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.