Open AccessMimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
Author(s) -
Michael Ansorge,
Jiranuwat Sapudom,
Marina Chkolnikov,
W.D. Martin,
Ulf Anderegg,
Stephanie Möller,
Matthias Schnabelrauch,
Tilo Pompe
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-05912-x
Subject(s) - paracrine signalling , myofibroblast , microbiology and biotechnology , transforming growth factor , chemistry , cellular differentiation , regulator , extracellular matrix , matrix (chemical analysis) , biology , fibrosis , pathology , medicine , receptor , biochemistry , gene , chromatography
TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGFβ1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGFβ1 release mimicking paracrine cell signals.