Open AccessHigh-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet
Author(s) -
Shihomi Masuda,
Yuhki Yanase,
Eiji Usukura,
Sou Ryuzaki,
Pangpang Wang,
Koichi Okamoto,
Thasaneeya Kuboki,
Satoru Kidoaki,
Kaoru Tamada
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-04000-4
Subject(s) - colloidal gold , nanoparticle , resolution (logic) , gold standard (test) , high resolution , nanotechnology , biomedical engineering , materials science , computer science , medicine , radiology , artificial intelligence , geology , remote sensing
This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.