Open AccessMorphologic and biometric evaluation of chick embryo eyes in ovo using 7 Tesla MRI
Author(s) -
Tobias Lindner,
Ronja Klose,
Felix Streckenbach,
Thomas Stahnke,
Stefan Hadlich,
JensPeter Kühn,
Rudolf Guthoff,
Andreas Wree,
Anne-Marie Neumann,
Marcus Frank,
Änne Glass,
Sönke Langner,
Oliver Stachs
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-02755-4
Subject(s) - in ovo , embryo , embryogenesis , biometrics , population , biology , anatomy , eye development , high resolution , biometric data , medicine , computer science , artificial intelligence , microbiology and biotechnology , genetics , environmental health , gene , phenotype , remote sensing , geology
The purposes of this study were (1) to characterize embryonic eye development during incubation in ovo and (2) to analyze the putative influence of repetitive ultrahigh-field MRI (UHF-MRI) measurements on this development. A population of 38 fertilized chicken eggs was divided into two sub-groups: two eggs (Group A) were examined repeatedly during the developmental period from embryonic day 1 (E1) to embryonic day 20 (E20) to evaluate the influence of daily MRI scanning. A second larger group of 36 eggs was examined pairwise on one day only, from E3 to E20, and the embryos were sacrificed immediately after MR imaging (Group B). Fast T2-weighted MR sequences provided biometric data on the eye with an in-plane resolution of 74 μm. The data show rapid growth of the eye with a steep increase in intraocular dimensions in all axis directions and in eyeball volume during initial development up to E10, followed by a phase of reduced growth rate in later developmental stages. Comparison of the two groups revealed no differences in ocular development.