Open AccessThe Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
Author(s) -
Filip Bartnicki,
Piotr Bonarek,
E. Kowalska,
Wojciech Strzałka
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-02432-6
Subject(s) - arginine , chemistry , cell , biochemistry , biology , computational biology , amino acid
The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.