Open AccessIdentification of autoreactive B cells with labeled nucleosomes
Author(s) -
Vincent Gies,
Alain Wagner,
Cécile Seifert,
Aurélien Guffroy,
Jean-Daniel Fauny,
Anne-M Knapp,
Jean-L Pasquali,
Thierry Martin,
Hélène Dumortier,
AnneSophie Korganow,
Pauline Soulas-Sprauel
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/s41598-017-00664-0
Subject(s) - autoantibody , nucleosome , flow cytometry , histone , biology , immunology , systemic lupus erythematosus , microbiology and biotechnology , autoimmunity , autoimmune disease , b cell , computational biology , antibody , dna , genetics , medicine , disease , pathology
The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.