Open AccessCRISPR RNA-guided autonomous delivery of Cas9
Author(s) -
Royce A. Wilkinson,
Coleman D. Martin,
Artem Nemudryi,
Blake Wiedenheft
Publication year - 2018
Publication title -
nature structural and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.448
H-Index - 270
eISSN - 1545-9993
pISSN - 1545-9985
DOI - 10.1038/s41594-018-0173-y
Subject(s) - crispr , cas9 , genome editing , computational biology , biology , rna , effector , guide rna , endonuclease , dna , genetics , gene , microbiology and biotechnology
Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing.