Open AccessAn adaptive optics module for deep tissue multiphoton imaging in vivo
Author(s) -
Cristina Rodríguez,
Anderson Chen,
José A. Rivera,
Manuel Mohr,
Yajie Liang,
Ryan G. Natan,
Wenzhi Sun,
Daniel E. Milkie,
Thomas G. Bifano,
Xiaoke Chen,
Na Ji
Publication year - 2021
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-021-01279-0
Subject(s) - somatosensory system , two photon excitation microscopy , spinal cord , multiphoton fluorescence microscope , calcium imaging , neuroscience , microscopy , microscope , preclinical imaging , adaptive optics , in vivo , fluorescence lifetime imaging microscopy , confocal , optics , fluorescence microscope , biology , fluorescence , materials science , physics , calcium , microbiology and biotechnology , metallurgy
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.