Open AccessClear optically matched panoramic access channel technique (COMPACT) for large-volume deep brain imaging
Author(s) -
Bowen Wei,
Chenmao Wang,
Zongyue Cheng,
Baoling Lai,
WenBiao Gan,
Meng Cui
Publication year - 2021
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-021-01230-3
Subject(s) - neuroimaging , volume (thermodynamics) , calcium imaging , preclinical imaging , optical imaging , biomedical engineering , brain tissue , materials science , optics , computer science , neuroscience , physics , in vivo , medicine , biology , calcium , microbiology and biotechnology , quantum mechanics , metallurgy
To understand neural circuit mechanisms underlying behavior, it is crucial to observe the dynamics of neuronal structure and function in different regions of the brain. Since current noninvasive imaging technologies allow cellular-resolution imaging of neurons only within ~1 mm below the cortical surface, the majority of mouse brain tissue remains inaccessible. While miniature optical imaging probes allow access to deep brain regions, cellular-resolution imaging is typically restricted to a small tissue volume. To increase the tissue access volume, we developed a clear optically matched panoramic access channel technique (COMPACT). With probe dimensions comparable to those of common gradient-index lenses, COMPACT enables a two to three orders of magnitude greater tissue access volume. We demonstrated the capabilities of COMPACT by multiregional calcium imaging in mice during sleep. We believe that large-volume in vivo imaging with COMPACT will be valuable to a variety of deep tissue imaging applications.