Open AccessL1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma
Author(s) -
Maia Norman,
Dmitry TerOvanesyan,
Wendy Trieu,
Roey Lazarovits,
Emma J. K. Kowal,
Ju Hyun Lee,
Alice ChenPlotkin,
Aviv Regev,
George M. Church,
David R. Walt
Publication year - 2021
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-021-01174-8
Subject(s) - extracellular vesicles , l1 , extracellular , cerebrospinal fluid , transmembrane protein , human plasma , vesicle , microbiology and biotechnology , extracellular fluid , chemistry , isolation (microbiology) , extracellular vesicle , biology , microvesicles , biochemistry , bioinformatics , neuroscience , membrane , microrna , gene , receptor , chromatography
L1CAM is a transmembrane protein expressed on neurons that was presumed to be found on neuron-derived extracellular vesicles (NDEVs) in human biofluids. We developed a panel of single-molecule array assays to evaluate the use of L1CAM for NDEV isolation. We demonstrate that L1CAM is not associated with extracellular vesicles in human plasma or cerebrospinal fluid and therefore recommend against its use as a marker in NDEV isolation protocols.